Scientific sessions, CRG Group Leader Seminars
Genome Biology Group, CNAG. Structural Genomics Group, CRG
I obtained a Ph.D. in Biophysics from the UAB where I worked on protein folding under the supervision of Professors B. Oliva, F.X. Avilés and M. Karplus. After that, I went to the US for a postdoctoral training on protein structure modeling at the Sali Lab (Rockefeller University) as the recipient of the Burroughs Wellcome Fund fellowship. Later on, I was appointed Assistant Adjunct Professor at UCSF. Between 2006 and 2011, I headed of the Structural Genomics Group at the CIPF in Valencia (Spain). Currently, I am ICREA research professor and lead the Genome Biology Group at the National Center for Genomic Analysis (CNAG) and the Structural Genomics Group at the Centre for Genomic Regulation (CRG), both in Barcelona. Our group is broadly interested on how RNA, proteins and genomes organize and regulate cell fate. I am Associate Editor of the PLoS Computational Biology and the BMC Structural Biology journals as well as have published over 80 articles in international peer-reviewed journal.
The genome three-dimensional (3D) organization plays important, yet poorly understood roles in gene regulation. Chromosomes assume multiple distinct conformations in relation to the expression status of resident genes and undergo dramatic alterations in higher order structure through the cell cycle. Despite advances in microscopy, a general technique to determine the 3D conformation of chromatin has been lacking. We developed a new method for the determination of the 3D conformation of chromatin domains in the interphase nucleus called TADbit (http://3DGenomes.org). The general approach of our method, which has been applied so far to study the 3D conformation of the alpha-globin domain in the human genome, the Caulobacter crescentus whole genome, and the dynamic response of Topologically Associating Domains (TADs) to hormone treatment in breast cancer cell lines, opens the field for comprehensive studies of the 3D conformation of chromosomal domains and contributes to a more complete characterization of genome regulation. During the presentation, I will update on the latest applications of TADbit for addressing biological questions on how genomes are organized in 3D.