20/01/2016 - 12:00 - Auditori PRBB

X-inactivation and X-reactivation: Investigating an epigenetic switch in mammalian development and pluripotency

Scientific sessions, CRG Group Leader Seminars

Bernhard Payer

Epigenetic Reprogramming in Embryogenesis and the Germline Group, Gene Regulation, Stem Cells and Cancer Programme, CRG

Female mammals inactivate one of two X-chromosomes to achieve equal gene dosage with males, which have only one X. This X-inactivation process includes multiple epigenetic mechanisms and is closely linked to the differentiated state. X-inactivation is reversed in pluripotent stem cells, during development and in the germ cell lineage by X-chromosome reactivation, which renders both X-chromosomes active.

Here I will present different candidate and unbiased screening approaches, which our laboratory undertakes to elucidate the mechanisms of the X-chromosome reactivation process. We study epigenetic reprogramming of the X-chromosome in mouse embryos, during induced pluripotent stem cell (iPSC) reprogramming and during formation of the germ cell lineage. Thereby we want to gain further insights into how X-reactivation is linked to its biological context and how the erasure of epigenetic modifications and gene reactivation is accomplished.

From October 2014 Group Leader, Centre for Genomic Regulation (CRG), Barcelona Spain
2006-2014 Postdoctoral work in laboratory of Jeannie Lee at Massachusetts General Hospital / Harvard Medical School, Boston, USA
2001-2006 Ph.D. in Developmental Biology in laboratory of Azim Surani at the Gurdon Institute, University of Cambridge, UK
2000-2001 M.Phil. in Developmental Biology, University of Cambridge, UK
1994-2000 Diploma in Biology/Genetics, University of Vienna, Austria