04/10/2012 - 09:00 - Auditori PRBB

Cell Culture Seminar


9:30 Registration of participants

10:00 Welcome

Speaker – Arancha Hernández - LGC Standards, Spain

10:05 Introduction IMIM. Speaker - Montserrat Torà - Directora Serveis Cientificotècnics IMIM

10:30 History of ATCC® and LGC Standards

Speaker: Nick Amiss - LGC Standards, ATCC Europe

A short history of the world’s most respected type culture collection.

10:45 Cell Culture Standards:
“Effects of Over-Passaging” & “Choosing and Using ATCC Cells”
Speaker: Nick Amiss
A great benefit after more than 100 years of cell culture is the vast number of cancer, primary and immortalised cell lines available to researchers. Using a panel of cancer cell lines will improve the robustness of results, but it is not straightforward which to choose. Primary cells have greater relevance to healthy human tissue, but culturing them is technically challenging. LGC offer tools to address these issues.

11:30 Coffee Break

11:45 Genetic and epigenetic dynamics in in vitro studies.
Speaker: Miguel A. Peinado, Institute of Predictive and Personalized Medicine of Cancer (IMPPC)

12:15 Evolution of Cell Culture and the Need for Cell Line Authentication
Speaker: Nick Amiss
The history of cell culture is a fascinating mix of black art and serendipity that has evolved over several decades into a precise science and indispensible tool. Yet this evolution is not without problems that remain prevalent to this day. Genetic and phenotypic drift contaminated and misidentified cell lines. Some very simple steps can be taken to prevent this from occurring and to authenticate a cell line's identity. This is a common requirement for publication in many oncology journals.

13:15 Panel Discussion: Question/Answer session
Opportunity for the audience to ask the presenters specific questions.

14:00 Lunch – End of program