30/09/2013 - 12:00 - PRBB

COURSES @ CRG: Applied Super-resolution Light Microscopy



Course Overview:
In the last years several methods have been developed to overcome the intrinsic limit of resolution in optical microscopy. These approaches comprise the field of super-resolution light microscopy.
It is the aim of this workshop to provide an overview of the current state of super-resolution methods and to give access to such instruments in the form of Stimulated Emission Depletion (STED) and localization microscopes. A special focus will be put on the following applied aspects of these techniques:
  • label properties and selection
  • optimization of imaging conditions
  • deconvolution of STED datasets
  • analysis/rendering of localization based data.
Resources available for the workshop:
  • Lecture rooms
  • Course room for data analysis session
  • Advanced Light Microscopy Unit (ALMU): currently 6 confocal microscopes (1x 2P, 1x 2P/FLIM/FCS, 1x CW-STED, 1 x spinning disk/bleaching/TIRF, 2x standard confocals), 1 GSD/Multiline TIRF microscope, 1 fully automated widefield microscope, supporting staff of four imaging specialists and one analysis specialist


  • María GARCÍA-PARAJO, The Institute of Photonic Sciences (ICFO), Barcelona, Spain
  • Melike LAKADAMYALI, The Institute of Photonic Sciences (ICFO), Barcelona, Spain
  • Thomas PENGO, CRG, Barcelona Spain
  • Giuseppe VICIDOMINI, Italian Institute of Technology, Genova, Italy
  • Katrin WILLIG, Max-Planck-Institute of Biophysical Chemistry, Goettingen, Germany
  • Timo ZIMMERMANN, CRG, Barcelona, Spain
  • Jordi ANDILLA, Institute of Photonic Sciences, Castelldefels, Spain
  • Raquel GARCIA, CRG, Barcelona, Spain
  • Arrate MALLABIABARRENA, CRG, Barcelona, Spain
  • Anna ODONE, Institute of Photonic Sciences, Castelldefels, Spain
  • Thomas PENGO, CRG, Barcelona, Spain
  • Xavier SANJUAN, UPF, Barcelona, Spain
  • Timo ZIMMERMANN, CRG, Barcelona, Spain
We only accept online registration. Registration is now closed.
The number of participants is limited to 20. The positions are open to candidates from institutes worldwide.
CRG (PRBB-Building)
Dr. Aiguader 88
08003 Barcelona
Contact person:
Eva Navarrete
CRG - Centre for Genomic Regulation
Dr. Aiguader, 88
08003 Barcelona, Catalonia, Spain
Tel.: +34 93 316 03 75