05/02/2014 - 12:00 - Auditori PRBB

Structure determination of genomes and genomic domains by satisfaction of spatial restraints

Scientific sessions, CRG Group Leader Seminars

Marc Martí Renom

Structural Genomics Group, Gene Regulation, Stem Cells and Cancer Programme, CRG. Centre Nacional d'Anàlisi Genòmica (CNAG)

Short biography:

Marc Martí-Renom obtained a Ph.D. in Biophysics from the UAB where he worked on protein folding under the supervision of Professors B. Oliva, F.X. Avilés and M. Karplus. After that, he went to the US for a postdoctoral training on protein structure modeling at the Sali Lab (Rockefeller University) as the recipient of the Burroughs Wellcome Fund fellowship. Later on, he was appointed Assistant Adjunct Professor at UCSF. Between 2006 and 2011, he was head of the Structural Genomics Group at the CIPF in Valencia (Spain). Currently, he is an ICREA research professor and he leads the Genome Biology Group at the National Center for Genomic Analysis (CNAG) and the Structural Genomics Group at the Centre for Genomic Regulation (CRG), both in Barcelona. His group is broadly interested on how RNA, proteins and genomes organize and regulate cell fate. He is an Associate Editor of the PLoS Computational Biology journal and has published over 70 articles in international peer-reviewed journal.

Talk summary:

The genome three-dimensional (3D) organization plays important, yet poorly understood roles in gene regulation. Chromosomes assume multiple distinct conformations in relation to the expression status of resident genes and undergo dramatic alterations in higher order structure through the cell cycle. Despite advances in microscopy, a general technique to determine the 3D conformation of chromatin has been lacking. We developed a new method for the determination of the 3D conformation of chromatin domains in the interphase nucleus, which combines 3C-based experiments with the computational Integrative Modeling Platform (IMP). The general approach of our method, which has been applied to study the 3D conformation of the alpha-globin domain in the human genome and the Caulobacter crescentus whole genome, opens the field for comprehensive studies of the 3D conformation of chromosomal domains and contributes to a more complete characterization of genome regulation. During the seminar, I will introduce our recent work on the structural response of Topologically Associating Domains (TADs) to hormone treatment in breast cancer cell lines.