CRG: 'Cut & Paste' is now for DNA dark matter too

CRG: 'Cut & Paste' is now for DNA dark matter too

News from CRG

For some time, scientists have been seeking a more efficient and reliable way to edit the genome and modify it to suit any need. A technique called CRISPR-Cas9 was recently launched as the solution to this problem, and has since taken a position as one of the most revolutionary techniques in molecular biology.

Although CRISPR-Cas9 is much more powerful than previous genome editing methods, it still has certain limitations. For example, it is very useful when dealing with genome fragments that code for proteins but, in reality, this covers just 1% of the genome. The remaining 99%, the "dark matter", or what was once known as "junk DNA" still could not benefit from the advantages offered by this revolutionary technique.

Now, researchers at the Centre for Genomic Regulation, led by Rory Johnson, are presenting a new method that makes it possible to use the CRISPR-Cas9 technique on DNA dark matter, too. The method has been presented in an article published in BMC Genomics and is available on an open-access basis for the entire scientific community.

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CRG News

Estel Aparicio-Prat, Carme Arnan, Ilaria Sala, Núria Bosch, Roderic Guigó and Rory Johnson. “DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs” BMC Genomics. 2015