News from DCEXS-UPF and CRG
New viruses present in human saliva have been isolated through the application of a technique that combine flow cytometry, genomics and molecular biology. This technique is called single-virus genomics (SVGs) and consists of separating a single virus by flow cytometry, breaking its capsid, making copies of the genome and sequencing its DNA to be able to identify it. This work published in the journal Viruses was leaded by Manuel Martínez, member of the University of Alicante, and has had the collaboration of Òscar Fornas, researcher of the UPF and head of the CRG Flow Cytometry Unit.
The technique was used with 15 volunteers and have managed to isolate 1,300 viruses and amplify the genome DNA in around 200 viruses, being discovered eight new viruses. Manuel Martínez clarifies that "we are focusing on comparing the changes that occur in these viruses, and also bacteria, present in the saliva of healthy and sick people with certain underlying diseases such as various immunodeficiencies". "The study is an example of the potential of this new technique to reveal genetic diversity of viruses in the human body since it could be implemented with any liquid sample," Oscar Fornas concluded. -CS/PRBB
Cruz MJ, Martinez-Hernandez F, Garcia-Heredia I, Lluesma M, Fornas O and Martinez-Garcia M. Deciphering the Human Virome with Single-Virus Genomics and Metagenomics. Viruses, March 2018.