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13/6/2014

IBE (CSIC-UPF): New 454 sequencing protocol from extremely small amounts of DNA

IBE (CSIC-UPF): New 454 sequencing protocol from extremely small amounts of DNA


News from IBE (CSIC-UPF)


In this study, researchers at the IBE and collaborators adjust the protocol for 454 sequencing for extremely small amounts of DNA. The authors demonstrate that their method beats whole genome amplification by multiple displacement amplification (aka GenomiPhi), and can be applied to metagenomics projects.

The study has been led by reserachers at the Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València in collaboration with IBE researchers Francesc Calafell and colleagues.

Reference:
Džunková M, Garcia-Garcerà M, Martínez-Priego L, D'Auria G, Calafell F, et al. (2014) Direct Squencing from the Minimal Number of DNA Molecules Needed to Fill a 454 Picotiterplate. PLoS ONE 9(6): e97379. doi:10.1371/journal.pone.0097379

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